Research projects

The constant realization of or visions and ideas, supported by publicly funded research, allows us to enhance our expertise and to improve our technological portfolio to be always up to speed of the fast growing microfluidic market.

Since the early beginning, BioFluidix was committed to participate or coordinate research projects together with partners from industry and research. A list of the performed projects follows below.

ProkOrA

Flexible production platform for customer specific single-use sample carriers for the point of care diagnostics especially for respiratory diseases und individualized medicine (ProkOrA)

Project description

The aim of this project is to develop production processes that enable an economic and flexible fabrication of plastic sample carriers for point of care diagnostics. That addresses an important technological gap in a multi-billion dollar growth-market.
The trend towards point of care diagnostics will be pushed if such sample carriers can be produced in a cost efficient and flexible way. Thus, therapeutic decisions can be made significantly faster and more efficient. As a result the mortality rates in hospitals will decrease and the unnecessary over-use of antibiotics will be reduced. In case of pandemics or emergency situations point of care diagnoses can be implemented at strategic transport hubs to dam the expansion.
Within the ProkOrA project those urgently required flexible processes to enable a stable reagent filling, precise spotting, reliable sealing and 100% quality control, will be developed

Project facts

Start 01.10.2015
Duration 24 months
Budget 1.5 Mio. €
Contact Dr.-Ing. Andreas Ernst

BioFluidix tasks

BioFluidix is contributing to this project through its knowhow and technology in the field of micro dispensing. BioFluidix will develop a novel dispensing unit which meets the needs for the pre-storage of reagents on the disks. Flexibility, accuracy and precision are a few keywords describing the task of BioFluidix.

Sponsors

Federal Ministry for Economic Affairs

ZIM-Program of German BMWI (Federal Ministry of Economic Affairs)

Projektträger Karlsruhe

Projektträger Karlsruhe (PTKA)

Research partners

altona Diagnostics GmbH

altona Diagnostics GmbH, Germany

biotechrabbit GmbH

biotechrabbit GmbH, Germany

Hahn-Schickard-Gesellschaft

Hahn-Schickard-Gesellschaft für angewandte Forschung e.V.

BioFluidix GmbH

BioFluidix GmbH, Germany

MOKAS
Lab Automation Network Logo

Development of a modular calibration system for pipetting-workstations (MOKAS)
The goal of the MOKAS project  is to develop an eight-channel optical volume calibration for pipetting workstations.

Project description

The MOKAS project aims to develop an eight channel camera system to calibrate pipetting systems using an optical feature recognition algorithm.

The footprint of the final calibration system is going to be size of a standard SBS-well plate. Further, the system will work autonomously as it is battery driven and will feature WiFi-communication. Thus, the calibration system can easily be integrated into existing pipetting workstations or dosage systems.

Project facts

Start 01.10.2015
Duration 24 months
Budget 246.000 €
Contact Andreas Madjarov,
e-mail: andreas.madjarov@biofluidix.com

BioFluidix tasks

Lab Automation Network Logo

BioFluidix is solely responsible for achieving the above mentioned results and for the realization of the project.

Sponsor

Federal Ministry of Economic Affairs

ZIM-Program of German BMWI (Federal Ministry of Economic Affairs)

Federal Ministry of Economic Affairs

ZIM-Program of German BMWI (Federal Ministry of Economic Affairs)

Research partners

BioFluidix GmbH

BioFluidix GmbH, Germany

SimPhoNy

Simulation framework for multi-scale phenomena in micro- and nanosystems (SimPhoNy)
The SimPhoNy project aims to develop an easy-to-use integrated multiscale modelling environment for in Silico discovery and design of nano-enabled systems and materials.

Project description

The SimPhoNy project aims to develop an easy-to-use integrated multiscale modelling environment for in Silico discovery and design of nano-enabled systems and materials. The main concept of the SimPhoNy framework is to augment existing open-source and commercial simulation tools and supplement them with sophisticated interface software libraries that allow for flow of information from one component to the other and from one scale to another. The integrated tools range from those describing the electronic structure and atomistic scales up to those modelling mesoscopic and macroscopic device level scales.
BioFluidix is particularly involved in the validation and evaluation of the SimPhoNy modelling environment with respect to the simulation of  micro- and nanofluidic process such as capillary phenomena, droplet formation, droplet spread, film formation and solid particle distribution in dried films. The SimPhoNy simulation tool will be another valuable asset for BioFluidix to better serve its customers with high-end science and knowledge based solutions for micro dosage, printing and coating applications.

Project facts

Start 01.01.2014
Duration 36 months
Budget 4 Mio. €
Website www.SIMPHONY-project.eu
Contact Dr. Peter Koltay,
e-mail: info@biofluidix.com

BioFluidix tasks

Definition of test cases for droplet spreading and drying on surfaces using complex, particle laden liquids. Measurement of rheological parameters of test liquids. Provision of experimental data of droplet generation, impact and drying by stroboscopic imaging using PipeJet® and / or TopSpot® printing technology. Evaluation of the SimPhoNy simulation platform for simulation of realistic printing and coating applications.

Sponsor

European Commission, Research Executive Agency

European Commission, Research Executive Agency (REA)

Research partners

Fraunhofer Institute

Fraunhofer Institute for Mechanics oh Materials, IWM, Germany

Enthought

Enthought, United Kingdom

Sophion BioScience

Sophion BioScience A/S, Denmark

Hellma GmbH

Hellma GmbH & CO. KG, Germany

MTT

MTT, Finland

Numerola Oy

Numerola Oy, Finland

Sgenia

Sgenia, Spain

Technion

Technion - Israel Institute of Technology, Israel

University of Jyväskylä

University of Jyväskylä, Finland

Quantech

Quantech ATZ, S.A., Spain

CIMNE

CIMNE, Spain

BioFluidix GmbH

BioFluidix GmbH, Germany

SICTEC
Lab Automation Network Logo

Single Cell Technologies for SMEs (SICTEC)
This project is focused on development of technologies for separation and manipulation of single biological cells for life science research and medical applications.

Project description

This project is focused on development of technologies for separation and manipulation of single biological cells for life science research and medical applications. The research plan is built upon the results of the FP7 project PASCA (GA 257073, started 01.09.2010). The single cell manipulation technology (SCM technology) developed there is based on inkjet-like printing of single biological cells confined in free flying micro droplets. It constitutes a universal platform for single cell analysis that has been proven to have high potential for many life science applications. The objective of this project is to support the participating SMEs and companies to take up the SCM technology, to realize their own applications and to develop them into innovative products for the medical, biomedical and pharmaceutical markets. Central element of the research is the SCM prototype instrument as presented in numerous publications (see www.pasca.eu). The actual project will provide validation and extension of the use of this prototype instrument and deal with necessary improvements and modifications of pre-production prototypes towards the specific needs and applications of the SME partners. In particular also topics affecting commercial exploitation like e.g. application development, design for manufacturability, reliability issues, cost and throughput optimization, extension of technical specifications, and preparation of CE IVD labeling will be investigated. Applications targeted by the individual SMEs are considering fast pathogen detection for clinical use by combining the SCM technology with MALDI TOF mass spectroscopy of single bacteria, instruments for single cell cancer and stem cell research, methods for monoclonal cell line development and in-vitro medical diagnostic applications. The anticipated innovations stemming from this research will be exploited by the involved SMEs individually as well as jointly through several innovative products targeted for different markets and applications.

Project facts

Start 01.10.2013
Duration 24 months
Budget 1.4 Mio. €
Website www.sictec-project.eu
Contact Dr. Wolfgang Streule,
e-mail: info@biofluidix.com

BioFluidix tasks

Lab Automation Network Logo

BioFluidix is coordinating the project and contributing to it through its NanoJetT® dispensing technology, system integration and software development.

Sponsor

European Commission

European Commission, Research Executive Agency (REA)

FP7

Seventh Framework Programme

Research partners

University of Freiburg

University of Freiburg, IMTEK, Germany

Erasmus MC

Erasmus MC, Netherlands

Sophion BioScience

Sophion BioScience A/S, Denmark

Primadiag

Primadiag SAS, France

INNOPROT

INNOPROT, Spain

BioSparQ

BioSparQ BV, Netherlands

BioFluidix GmbH

BioFluidix GmbH, Germany

Selected publications

A Non-Invasive Single Cell Dispensing Approach for 2-Dimensional Micro-Patterning,
A. Yusof, L. Riegger, N. Paust, A. Ernst, R. Zengerle, P. Koltay Actuator 2010, Bremen, Germany, 14.-16. June, 2010, 1033 - 1036

Towards a microfluidic dispenser chip for printing of single cells,
A. Yusof, R. Zengerle, and P. Koltay Proc. of IEEE-MEMS, Cancun, Mexico, January 23-27, 2011, 1059-1062

Inkjet-like printing of single-cells,
A. Yusof, H. Keegan, C. D. Spillane, O. M. Sheils, C. M. Martin, J. J. O'Leary, R. Zengerle and P. Koltay Lab Chip, 2011, Advance Article, DOI: 10.1039/C1LC20176J

E-Dosis

E-Dosis - Einweg Dosiersystem mit intelligenter Sensorik
The project aims to develop a disposable sensor controlled dosing system which can dispense fluids independent of the rheological properties. BioFluidix is contributing the electronics for the flow sensor and the dispensing valve to this project.

Project description

The project aims to develop a disposable sensor controlled dosing system which can dispense fluids independent of the rheological properties.  The technology bases on a disposable electromagnetic pressure/time controlled dispensing valve for the sub-µl range which is combined with a flow sensor and a real-time control in order to achieve a high precision and accuracy. For this purpose a disposable capacitive flow sensor will be developed which can measure small flow rates by means of a differential pressure method.

Project facts

Start 01.07.2013
Duration 36 months
Budget 0.99 Mio. €
Website will be launched soon
Contact Dr. Andreas Ernst,
e-mail: info@biofluidix.com

BioFluidix tasks

BioFluidix is contributing the electronics for the flow sensor and the dispensing valve to this project and furthermore assists in the integration of the disposable parts into the periphery and the dosing system.

Sponsor

BMBF

BMBF

Research partners

University of Freiburg

University of Freiburg, IMTEK, Germany

Braunform GmbH

Braunform GmbH, Germany

BioFluidix GmbH

BioFluidix GmbH, Germany

KombiFun
Lab Automation Network Logo

KombiFun - Entwicklung eines Ressourcenschonenden Kombinationsverfahrens zur Herstellung ortsselektiver elektronischer Funktionsschichten

Aim of the project is the development of a combined printing and laser sintering process to selectively fabricate conductive or isolating functional layers on target substrates.

Project description

Aim of the project is the development of a combined printing and laser sintering process to selectively fabricate conductive or isolating functional layers on target substrates. Due to the selective deposition, the amount of material specifically precious metals used during processing can be greatly reduced. The end user Limitor defines the specifications of the process as well as materials and deposited layers and tests the fabricated samples. FEW chemicals develops the base solutions for conductive and respectively isolating layers and conducts optimizations in respect to printing as well as laser treatment properties. BioFluidix develops a printhead with integrated means to reduce sedimentation in particle dispersions and optimizes the printing process. Fraunhofer ILT will develop the laser sintering process for post-processing the functional and patterned substrates.

Project facts

Start 01.10.2012
Duration 24 months
Budget 1.1 Mio. €
Contact Dr. Lutz Riegger,
e-mail: info@biofluidix.com

BioFluidix tasks

BioFluidix is contributing the printing equipment and process development for the deposition of isolation layers and metal particle dispersions.

Sponsor

BMBF

BMBF

Research partners

Fraunhofer ILT

Fraunhofer ILT, Germany

FEW Chemicals

FEW Chemicals GmbH, Germany

Limitor GmbH

Limitor GmbH, Germany

BioFluidix GmbH

BioFluidix GmbH, Germany

PASCA
Lab Automation Network Logo

PASCA - Platform for Advanced Single Cell Manipulation and Analysis

The central theme of the project is the printing of single biological cells confined in micro-droplets of only one tenth of a millimetre in diameter. The mid-term objective is to establish a platform for the manipulation, culture, and analysis of individual biological cells without loss of viability.

Project description

The central theme of the project is the printing of single biological cells confined in micro-droplets of only one tenth of a millimetre in diameter. The mid-term objective is to establish a platform for the manipulation, culture, and analysis of individual biological cells without loss of viability.

Project facts

Start 01.09.2010
Duration 36 months
Budget 3 Mio. €
Website www.pasca.eu
Contact Dr. Wolfgang Streule,
e-mail: info@biofluidix.com

BioFluidix tasks

Lab Automation Network Logo

BioFluidix is contributing to this project through its NanoJet® dispensing technology, system integration and software development.

Sponsor

European Commission

European Commission, Research Executive Agency (REA)

FP7

Seventh Framework Programme

Research partners

University of Freiburg

University of Freiburg, IMTEK, Germany

Sophion BioScience

Sophion BioScience A/S, Denmark

University of Dublin

University of Dublin, Trinity College, Ireland

Primadiag

Primadiag SAS, France

Zurich Instruments

Zurich Instruments AG, Switzerland

INNOPROT

INNOPROT, Spain

BioFluidix GmbH

BioFluidix GmbH, Germany

Selected publications

A Non-Invasive Single Cell Dispensing Approach for 2-Dimensional Micro-Patterning,
A. Yusof, L. Riegger, N. Paust, A. Ernst, R. Zengerle, P. Koltay Actuator 2010, Bremen, Germany, 14.-16. June, 2010, 1033 - 1036

Towards a microfluidic dispenser chip for printing of single cells,
A. Yusof, R. Zengerle, and P. Koltay Proc. of IEEE-MEMS, Cancun, Mexico, January 23-27, 2011, 1059-1062

Inkjet-like printing of single-cells,
A. Yusof, H. Keegan, C. D. Spillane, O. M. Sheils, C. M. Martin, J. J. O'Leary, R. Zengerle and P. Koltay Lab Chip, 2011, Advance Article, DOI: 10.1039/C1LC20176J

PipeJetTip
PipeJetTip

PipeJetTip - Pipetting technology for the next generation of diagnostics products

The project aims to develop a disposable pipette tip and a unique, innovative dispensing technology.

PipeJetTip

Project description

The project aimed to develop a disposable pipette tip and a unique, innovative dispensing technology (= technology for the next generation of diagnostic products) to establish a new standard in pipetting of volumes in the nanoliter range.

Project facts

Start 01.09.2010
Duration 28 months
Budget 600.000 €
Website www.microtec-suedwest.de
Contact Dr. Wolfgang Streule,
e-mail: info@biofluidix.com

BioFluidix tasks

Lab Automation Network Logo

BioFluidix contributed the electronics for the actuator to this project and furthermore assisted in the integration into existing laboratory equipment.

Sponsor

MicroTEC Südwest

BMBF, vertreten durch VDI/VDE-IT und MicroTEC Südwest (Spitzencluster)

BMBF

BMBF

Research partners

University of Freiburg

University of Freiburg, IMTEK, Germany

Brand

Brand GmbH & Co. KG, Germany

BioFluidix GmbH

BioFluidix GmbH, Germany

MMP
Lab Automation Network Logo

MMP - Micro Master Printer

The aim of the MMP project is the development of a rapid prototyping technology to produce porous micro masters in an additive process, based on direct printing of liquid metal.

Project description

The aim of the MMP project is the development of a rapid prototyping technology to produce porous micro masters in an additive process, based on direct printing of liquid metal. The ejection of single liquid metal micro droplets, with sizes in the low micrometer scale (D >= 40 µm), is realized by the application of the so called ‘StarJet’ Technology. A star shaped silicon nozzle allows for the production of single liquid metal droplets driven by a simple pneumatic pulse, which is needless of mechanical interaction between the liquid metal and the print-head.  This technology enables to produce porous, three dimensional metal structures by the agglomeration of single liquid metal droplets, printed at their individual target position.  Beside the production of master structures applied for hot embossing of microfluidic devices, this technology can be applied in a variety of different fields of applications e.g. dental production.

Project facts

Start 01.02.2010
Duration 39 months
Budget 320.000 €
Website www.voxeljet.de/mmp
Contact Dr. Andreas Ernst,
e-mail: info@biofluidix.com

BioFluidix tasks

BioFluidix is contributing in the design of the printhead, the passivation of the silicon nozzles as well as the development of the driving electronics of the printing system, firm- and software. Furthermore the printhead was installed on a BioFluidix iBioSpot platform to realize the 3D printing of the first metal structures.

Sponsor

ERANET

ERANET

Research partners

University of Freiburg

University of Freiburg, IMTEK, Germany

voxeljet AG

voxeljet AG, Germany

Rohrer AG

Rohrer AG, Switzerland

Inspire AG

Inspire AG, Switzerland

BioFluidix GmbH

BioFluidix GmbH, Germany

FunLas
Lab Automation Network Logo

FunLas - Functionalization of thin, nanoparticle-based layers on susceptible steel surfaces via brilliant diode laser radiation

The project "FunLas" aimed for the development of a cost- and resource efficient, laser-based method to fabricate mechanically robust wear protective coatings for the application on components experiencing high stress, e.g. motor or transmission parts.

Project description

The project "FunLas – Functionalization of thin, nanoparticle-based layers on susceptible steel surfaces via brilliant diode laser radiation" aimed for the development of a cost- and resource efficient, laser-based method to fabricate mechanically robust wear protective coatings for the application on components experiencing high stress, e.g. motor or transmission parts.

Due to the intensive collaboration between partners it was possible to create a solid basis for reaching this goal. Further, the aimed for process chain was successfully demonstrated on a laboratory level.

Project facts

Start 01.09.2008
Duration 36 months
Budget 3.2 Mio. €
Contact Dr. Lutz Riegger
e-mail: info@biofluidix.com

BioFluidix tasks

BioFluidix is contributing to this project through its PipeJet® printing technologies, i.e. the wet chemical coating of stainless steel motor parts.

Sponsor

BMBF

BMBF

Research partners

SchaefflerKG

SchaefflerKG (Coordinator), Germany

Merck KgaA

Merck KgaA, Germany

Fraunhofer Institut für Lasertechnik

Fraunhofer Institut für Lasertechnik, Germany

DILAS Diodenlaser GmbH

DILAS Diodenlaser GmbH, Germany

BioFluidix GmbH

BioFluidix GmbH, Germany

AUTOCAST
Lab Automation Network Logo

AUTOCAST - Automated Cancer Screening based on real-time PCR

In this project, a novel low cost automated real "point of care" diagnostic device is developed for both clinical and laboratory use. The technology is based on a microfluidic disposable cartridge enabling real time polymerase chain reaction (PCR) amplification and detection.

Project description

In this project, a novel low cost automated real “point of care” diagnostic device is developed for both clinical and laboratory use. The technology is based on a microfluidic disposable cartridge enabling real time polymerase chain reaction (PCR) amplification and detection. The disposable cartridge will be processed by a small integrated low cost thermo-cycler and reader system which can perform the diagnostic test automatically. The diagnostic test relies on a panel of biomarkers, which are detected on a microarray to allow for multi parameter analysis. The detection is based on the most sensitive TIRF method (TIRF = total internal reflection fluorescence). By this, the sensitivity and specificity of the detection can be improved and quantitative results can be achieved.

Project facts

Start 01.08.2008
Duration 42 months
Budget 4.1 Mio. €
Website http://www.genoid.net/autocast/
Contact Dr. Peter Koltay,
e-mail: info@biofluidix.com

BioFluidix tasks

BioFluidix is contributing to this project through its TopSpot® and PipeJet® printing technologies and expertise in microfluidic system design.

Sponsor

European Commission

European Commission, Research Executive Agency (REA)

FP7

Seventh Framework Programme

Research partners

GenoID Ltd.

GenoID Ltd. (Coordinator), Hungary

Jenoptik Polymer Systems GmbH

Jenoptik Polymer Systems GmbH, Germany

Fraunhofer Institute for Physical Measurement Techniques

Fraunhofer Institute for Physical Measurement Techniques, Germany

Austrian Research Centers GmbH

Austrian Research Centers GmbH, Austria

University of Freiburg

University of Freiburg, IMTEK, Germany

Trinity College, Dublin

Trinity College, Dublin and TheCoombe Women’s Hospital, Ireland

BioFluidix GmbH

BioFluidix GmbH, Germany

Selected publications

Adhesive bonding of microfluidic chips: influence of process parameters
Lutz Riegger, Journal of Micromechanics and Microengineering, 2010

Histology of cervical intraephihelial neoplasia and the role of biomarkers
Cara M. Martin, Best practise and research, Clinical obstretrics & gynaecology, 05/2011, 605-615

MicroActive
Lab Automation Network Logo

MicroActive - Automatic Detection of Disease Related Molecular Cell Activity

The MicroActive project has developed an instrument for molecular diagnostics intended for use in the doctors’ office. The instrument will first be used for patient screening for a group of viruses causing cervical cancer.

Project description

The MicroActive project has developed an instrument for molecular diagnostics intended for use in the doctors’ office. The instrument will first be used for patient screening for a group of viruses causing cervical cancer. Microfluidics and biotechnology form the basis for the development.

Project facts

Start 01.11.05
Duration 36 months
Budget 2.7 Mio. €
Website http://www.sintef.no/Projectweb/Microactive
Contact Dr. Peter Koltay,
e-mail: info@biofluidix.com

BioFluidix tasks

Lab Automation Network Logo

BioFluidix is contributing to this project through its PipeJet® printing technology and microfluidic design experience.

Sponsor

European Commission

European Commission

Research partners

SINTEF

SINTEF (Coordinator), Norway

University of Freiburg

University of Freiburg, IMTEK, Germany

NorChip AS

NorChip AS, Norway

IMM GmbH

IMM GmbH, Germany

Coombe Women & INfants Universitiy Hospital

Coombe Women & INfants Universitiy Hospital, Ireland

BioFluidix GmbH

BioFluidix GmbH, Germany

Selected publications

http://www.sintef.no/Projectweb/Microactive/Publications/

InnoNet

InnoNet - Venenklappe
The focus of the project was the development of a percutaneously implantable venous valve prosthesis.

Project description

The focus of the project was the development of a percutaneously implantable venous valve prosthesis. The department "Orthopaedics and motion systems" of FhG-IPA in collaboration with industry partners and the RWTH Aachen developed a novel 3D printing technology to produce vein valve prosthese models from PCU. These valves are only a few millimeters in size and can be used to replace the damaged vein valves own.

Project facts

Start 01.10.2007
Duration 36 months
Budget 802.000 €
Website http://www.vdivde-it.de/innonet/projekte/pz/in_pp201_venenklappe.pdf
Contact Dr. Wolfgang Streule,
e-mail: info@biofluidix.com

BioFluidix tasks

Lab Automation Network Logo

BioFluidix contributed the dispensing technology to dispense nl-amounts of PCU dissolved in aggressive solvents like DMA.

Sponsor

VDI/VDE-IT

VDI/VDE-IT

BMBF

BMBF

Research partners

VDI/VDE-IT

VDI/VDE-IT