Protein crystallization

Protein crystallography experiments can be time consuming and expensive. The automation allows you to increase the reproducibility of your experiments to get stable, comparable restults. The handling of low-volumes helps to minimize the consumption of expensive material.

Save expansive reagent

By aspiration of your samples from a source plate, only a very small quantities of stock is used for the transfer to the protein crystallogrphy plate. The dead volulme of PipeJet®  pipes is only a few µl!

Sitting drop

Transfer small nanoliter volumes by dispensing single droplets in the range of 2 - 70 nanoliters precisely on crystallography plates.

  • Reduce reagent costs
  • Increase crystallization runs
  • Prevent the risk of contamination
  • Reproducible and comparable experimental results

Relevant literature

On-demand droplet loading of ultrasonic acoustic levitator and its application for protein crystallography experiments | 2019

In this Letter, we explore a method of loading small droplets by using DoD dispensers to eject droplets and capturing those by the acoustic levitator. By analyzing the required condition for the trapping, we have developed a stable trapping strategy and applied the method to conduct a pilot protein crystallography experiment. In this way, we showed that the combination of the DoD dispensing method with the ALD is promising to realize an automated X-ray diffraction experiment without solid containers for crystals.

Read the article